Micropreparative separation of peptides derived from sodium dodecyl sulphate-solubilized proteins
Summary
Type: HPLC Application #: 108180
Detection: UV, 215nm Conditions: eluent A: 0.1% trifluoroacetic acid (TFA) in water, eluent B: 0.07% TFA in acetonitrile, linear gradient from 0 to 60% B in 60min
flow rate 100µl/min Authors: Bosserhoff A Source: J Chromatogr 473 (1989) 71 - 77
Details
Legend: Fig. 1. HPLC of tryptic peptides (200pmol) derived from bovine serum albumin,
sample A: no SDS, samples B and C: 50µg SDS, C treated with heptane - isoamyl
alcohol (4:1, v/v) before injection.